DOES LEUCINE-ENRICHED PROTEIN REALLY AFFECT PROTEIN LEVELS IN THE MUSCLES OF OLD MICE?

Yu. S. Onufrieva; O.M. Hasiuk; S.P. Beschasnyi

doi:10.32999/ksu2524-0838/2025-37-5

Yu. S. Onufrieva
O.M. Hasiuk
S.P. Beschasnyi

DOI: https://doi.org/10.32999/ksu2524-0838/2025-37-5

Ключові слова: insulin, leucine, sarcopenia, muscle aging, whey protein

Анотація

Sarcopenia, characterised by the progressive loss of skeletal muscle mass, strength, and functionality, represents a significant health concern in the aging population. One of the promising approaches to preventing sarcopenia is the use of the amino acid leucine, which activates the mTOR signalling pathway and promotes muscle protein synthesis. Reduced muscle strength and coordination increase the risk of falls, which can lead to severe injuries, such as hip or vertebral fractures. Such injuries often require prolonged recovery and may result in disability. Sarcopenia is often accompanied by an increase in fat mass against the background of muscle loss. This reduces the basal metabolic rate, contributing to the development of obesity and metabolic diseases such as type 2 diabetes. Loss of muscle mass lowers overall physical endurance, complicating the function of the heart and blood vessels, which increases the risk of cardiovascular diseases and related complications. The study was conducted on aged mice (25 months old), divided into three groups: a control group, a group receiving pure leucine supplementation, and a group receiving leucine-enriched whey protein. At the end of the experiment, the levels of leucine, glucose, and insulin in the blood, as well as protein synthesis in muscle tissue, were assessed. The results showed that the administration of pure leucine significantly increased its concentration in the blood (8-fold) and muscle tissue (3.8-fold) but did not promote muscle protein synthesis. In contrast, administering leucine in a whey protein mixture showed a less pronounced increase in leucine levels but effectively enhanced protein synthesis (by 1.2-fold) and reduced glucose levels. Insulin levels increased in both experimental groups, but this effect was less pronounced in the leucine mixture group. These findings underscore the potential efficacy of using leucine in combination with whey protein as an effective strategy for maintaining muscle mass and function during ageing. The obtained data have practical significance for the development of dietary and pharmacological strategies in combating sarcopenia.

Посилання

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