• H. Tkaczenko
  • O. Tverdokhlib
  • V. Honcharenko
  • N. Kurhaluk
Ключові слова: European mistletoe (Viscum album L.), extracts, rainbow trout (Oncorhynchus mykiss Walbaum, 2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives of oxidative modification of proteins, total antioxidant capacity (TAC)


Many studies, based on the understanding of the relationship between the generation of reactive oxygen species and antioxidant defences, propose a link with the recent use of mistletoe based on its antioxidant properties, supported by phytochemical and pharmacological data. The uniqueness of mistletoe metabolism, a direct consequence of its hemiparasitism, is used as a key interpretive element to explain its biological properties and guide its consequent therapeutic use.The aim of the present study was to determine the antioxidant activity of extracts derived from the leaves of Viscum album L. using biomarkers of oxidative stress [2-thiobarbituric acid reactive substances (TBARS) as a biomarker of lipid peroxidation, carbonyl derivatives of oxidative modification of proteins, total antioxidant capacity (TAC)] in the muscle tissue of rainbow trout (Oncorhynchus mykissWalbaum) after in vitro treatment with extracts at two final concentrations (5 and 2.5 mg/mL). Our study revealed that treatment with V. album leaf extracts resulted in non-significant changes in TBARS levels in the muscle tissue after in vitro incubation with V. album leaf extracts at final concentrations of 5 and 2.5 mg/mL.The levels of aldehydic derivatives of oxidatively modified proteins in rainbow trout muscle tissue after treatment with V. album leaf extracts at final concentrations of 5 mg/mL were at the same levels as in untreated controls. When muscle tissue was incubated with V. album leaf extracts, the levels of ketonic derivatives were significantly reduced after treatment with extracts at a final concentration of 5 and 2.5 mg/mL compared to untreated samples. TAC levels in rainbow trout muscle tissue were increased after in vitro incubation with V. album leaf extracts (at final concentrations of 5 and 2.5 mg/mL) compared to untreated samples. The results of the current study demonstrated the antioxidant properties of extracts from the leaves of V. album at two final concentrations (5 and 2.5 mg/mL) after incubation with rainbow trout muscle tissue.Further studies should focus on the antioxidant effect of extracts derived from the leaves of Viscum album using other cellular models.


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