• H. Tkaczenko
  • M. Opryshko
  • І. Antonik
  • M. Maryniuk
  • O. Gyrenko
  • L. Buyun
  • N. Kurhaluk
Ключові слова: commercial rosemary essential oil, antibacterial properties, inhibition zones, Kirby-Bauer disc diffusion technique


Rosemary essential oil has many compounds suitable for use in the food, cosmetic and pharmaceutical industries. Rosemary essential oils are widely used in the treatment of upper and lower respiratory tract diseases, mainly due to their antibacterial and antiviral effects.This study provides insight into the in vitro antibacterial activity of commercial rosemary essential oil against Gram-negative strains such as Escherichia coli(Migula) Castellani and Chalmers (ATCC® 25922™), E. coli (Migula) Castellani and Chalmers (ATCC® 35218™), Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 27853™) and Gram-positive strains such as Staphylococcus aureus subsp. aureusRosenbach (ATCC® 29213™), methicillin-resistant (MRSA) S. aureus (NCTC® 12493), Enterococcus faecalis(Andrewes and Horder) Schleifer and Kilpper-Balz(ATCC®51299™) (resistant to vancomycin; sensitive to teicoplanin) and E. faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz(ATCC®29212™). The results of the current study showed that Gram-negative bacterial strains such as E. coli (Migula) Castellani and Chalmers (ATCC® 35218™) and P. aeruginosa (Schroeter) Migula (ATCC® 27853™) were resistant to REO. The diameters of the inhibition zones after application of REO were similar to those of the control samples (96% ethanol). The increase in inhibition zone diameter after application of REO was 32.6% (p < 0.05) for Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) strains compared to control samples (96% ethanol). Similarly, the increase in inhibition zone diameters after application of REO was 50.3% (p < 0.05) for Gram-positive strains such as S. aureus subsp. aureusRosenbach (ATCC® 29213™).Methicillin-resistant S. aureus (NCTC® 12493)was resistant to REO.On the other hand, the largest inhibition zone diameters after application of REO were observed for E. faecalis strains.The increase in inhibition zone diameters after application of REO was 115.5% (p < 0.05) and 115.8% (p < 0.05) for E. faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299™) and E. faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 29212™) strains, respectively. The results suggest that commercial rosemary essential oil supplied by a Polish essential oil manufacturer (NaturalneAromaty sp. z o.o., Kłaj, Poland) has some significant antimicrobial properties. In vivo studies are needed to calculate the effective dose of EOs and to determine their possible side effects and toxicity.


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