• H. Tkaczenko
  • I. Aksonov
  • I. Tkachova
  • N. Kurhaluk
Ключові слова: English half-breed horses, exercise, blood, 2-thiobarbituric acid reactive substances (TBARS), carbonyl groups of oxidatively modified proteins, total antioxidant capacity (TAC)


 Physical exertion and exercise-induced stress in horses vary according to the equestrian discipline and breed. The correct training programme aims to adapt the horse's organism to the physiological changes that occur during intense physical exertion. The aim of our research was to investigate the effect of physical training on the levels of markers of oxidative stress [2-thiobarbituric acid reactive substances (TBARS), carbonyl groups of oxidatively modified proteins, total antioxidant capacity (TAC)] in the peripheral blood of healthy English half-breed horses. Nine healthy English half-breed horses (7 mares and 8 stallions) living in the village of Karlikowo, in the administrative district of GminaKrokowa, within the Puck district, Pomeranian Voivodeship, in northern Poland were used in this study. Blood was collected from the animals' jugular veins in the morning, 90 min after feeding, while the horses were in the stable and immediately after exercise. The training started at 10:00 a.m., lasted 1 hour and consisted of a cross-country ride at a walk (5 mins), a trot (15 mins), a walk (10 mins), a trot (10 mins), a walk (5 mins), a canter (5 mins) and a walk (10 mins).The results of our study showed that blood levels of TBARS, as a biomarker of lipid peroxidation, showed a non-significant change in both mares and stallions immediately after exercise compared to the resting period. Blood levels of aldehydic and ketonic derivatives of oxidatively modified proteins were reduced in both mares and stallions after exercise compared to pre-exercise levels. These reductions were statistically significant. In the blood of mares and stallions, TAC levels were increased after exercise compared to pre-exercise levels. This increase was not statistically significant. The results of our study showed that exercise did not induce oxidative stress in the blood of English half-breeds mares and stallions. These results would provide valuable information for understanding the adaptation of horses to exercise.


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