BIOMARKERS OF OXIDATIVE STRESS AND ANTIOXIDANT DEFENSES IN THE BLOOD OF SEA TROUT (SALMO TRUTTA M. TRUTTA L.) AFFECTED BY ULCERATIVE DERMAL NECROSIS SYNDROM
Анотація
Key words: sea trout, Salmo trutta m. trutta L., ulcerative dermal necrosis syndrome, oxidative stress, antioxidant defense system.
Antioxidant defense system (activities of superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase, ceruloplasmin content, total antioxidative capacity), and oxidative stress biomarkers were determined in the blood of sea trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis (UDN) syndrome. In both males and females, lipid and protein oxidation in the blood from UDN-affected trout showed higher values as compared to the respective healthy specimens. The UDN syndrome induced an increase of thiobarbituric acid reactive substances (TBARS) levels only in the plasma and decrease in blood catalase activity. Both the glutathione-mediated antioxidant defense system and endogenous SOD play a critical role in intracellular antioxidant defense against UDN-induced oxidative stress. This study encourages efforts to extend the knowledge of oxidative stress biomarkers for the identification of Aeromonas induced disorders and specific responses of fish typical of the UDN syndrome.
Посилання
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8. Halaktionova L.P., Molchanov A.V., Elchaninova S.A., Varshavskiy B.Ya. The lipid peroxidation processes in patients with ulcerous illness of stomach and duodenum // Clin. Lab. Diagnostics. - 1998. - Vol. 6. - P. 10-14 (In Russian).
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17. Kurhalyuk N., Tkachenko H. Induction of oxidative stress and antioxidant defenses in the liver of sea trout, Salmo trutta L., with ulcerative dermal necrosis // Arch. Pol. Fish. -2011. - Vol. 19. - P. 229-240.
18. Kurhalyuk N., Palczynska K., Tkachenko H. Oxidative stress and antioxidant defenses in reproductive organs of brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis // 25th Congress of the Polish physiological society. September 15-17,
2011, Olsztyn, Poland / In: J. Physiol. Pharmacol. - 2011. - 62(Suppl. 1). - P. 147.
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20. Kurhalyuk N., Tkachenko H., Palczynska K. Antioxidant enzymes profile in the brown trout (Salmo trutta trutta) with ulcerative dermal necrosis // Bull. Vet. Inst. Pulawy. -2009. - Vol. 53. - P. 813-818.
21. Kurhalyuk N., Tkachenko H., Palczynska K. Lipid peroxidation and antioxidant defense system in spawn of brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis // Arch. Pol. Fish. - 2010. - Vol. 18. - P. 115-122.
22. Kurhalyuk N., Tkachenko H., Palczynska K. Resistance of erythrocytes from brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome // Pol. J. Vet. Sciences. - 2011. - Vol. 14(3). - P. 443-448.
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28. Munro A.L.S. Ulcerative dermal necrosis, a disease of migratory salmonid fishes in the rivers of the British Isles // Biol. Conserv. _ 1970. _ Vol. 2. _ P. 129_132.
29. Noga E.J. Skin ulcers in fish: Pfiesteria and other etiologies // Toxicol. Pathol. _ 2000. _ Vol. 28(6). _ P. 807_823.
30. Pryor W.A. Oxy-radicals and related species: their formation, lifetimes and reactions. Annu. Rev. Physiol. _ 1986. _ Vol. 48. _ P. 657_667.
31. Ravin H.A. An improved colorimetric enzymatic assay of ceruloplasmin // J. Lab. Clin. Med. _ 1961. _ Vol. 58. _ P. 161_168.
32. Rehulka J. Aeromonas causes severe skin lesions in rainbow trout (Oncorhynchus mykiss): clinical pathology, haematology and biochemistry // Acta Vet. Brno. _ 2002. _ Vol. 71. _ P. 351_360.
33. Roberts R.J. Ulcerative dermal necrosis (UDN) in wild salmonids // Fish Res. _ 1993. — 17. _ P. 3_14.
34. Sagara Y., Dargusch R., Chambers D., Davis J., Schubert D., Maher P. Cellular mechanisms of resistance to chronic oxidative stress // Free Radic. Biol. Med.. _ 1998. _ Vol. 24. _ P. 1375_1389.
35. Sohal R.S. Role of oxidative stress and protein oxidation in the aging process. Free Radic. Biol. Med. _ 2002. _ Vol. 33. _ P. 37_44.
36. Stadtman E.R., Levine R.L. Protein oxidation // Ann. N. Y. Acad. Sci. _ 2000. _ Vol. 899. _P. 191_208.
37. Szewczyk E.: Bacteriological diagnostic // Warszawa, PWN (In Polish). _ 2005.
38. Tkachenko H., Kurhalyuk N., Palczynska K. Responses of antioxidant status in the gills of brown trout (Salmo trutta m. trutta L.) // J. Ecol. Prot. Coastline. _ 2011. _ Vol. 15. _ P. 149_162.
39. Ursini F., Bindoli A. The role of selenium peroxidases in the protection against oxidative damage of membranes. Chem. Phys. Lipids. _ 1987. _ Vol. 44. _ P. 255_276.
40. Valavanidis A., Vlahogianni T., Dassenakis M., Scoullos M. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants // Ecotoxicol. Environ. Saf. _ 2006. _ Vol. 64. _ P. 178_189.
41. Wdzieczak J., Zalesna G., Wujec E., Peres G. Comparative studies on superoxide dismutase, catalase and peroxidase levels in erythrocytes and livers of different freshwater and marine fish species // Comp. Biochem. Physiol. B. _ 1982. _ Vol. 73. _ P. 361_365.
42. Zar J.H. Biostatistical Analysis. 4th ed., Prentice-Hall Inc., Englewood Cliffs, New Jersey. _ 1999.
2. Bagnyukova T.V., Chahrak O.I., Lushchak V.I. Coordinated response of goldfish antioxidant defenses to environmental stress // Aquatic Toxicol. - 2006. - Vol. 78. - P. 325-331.
3. Bruno D., Crumlish M., LaPatra S., Noguera P., Verner-Jeffreys D. Workshop on salmonid skin diseases. European Association of Fish Pathologists 13th International Conference on Fish and Shellfish Diseases, Grado, Italy, 18th September. - 2007. -P. 7-10.
4. Cadenas E., Hochstein P., Ernster L. Pro- and antioxidant functions of quinones and quinone reductases in mammalian cells // Adv. Enzymol. - 1992. - Vol. 65. - P. 97-146.
5. Dorval J., Hontela A. Role of glutathione redox cycle and catalase in defense against oxidative stress induced by endosulfan in adrenocortical cells of rainbow trout (Oncorhynchus mykiss) // Toxicol. Appl. Pharmacol. - 2003. - Vol. 192. - P. 191-200.
6. Dubinina E.E., Burmistrov S.O., Khodov D.A., Porotov I.G. Oxidative modification of human serum proteins. A method of determining it // Vopr. Med. Khim. - 1995. - Vol. 41. - P. 24-26 (In Russian).
7. Glatzle D., Vuilleumier J.P., Weber F., Decker K. Glutathione reductase test with whole blood, a convenient procedure for the assessment of the riboflavin status in human // Experientia. - 1974. - Vol. 30. - P. 665-667.
8. Halaktionova L.P., Molchanov A.V., Elchaninova S.A., Varshavskiy B.Ya. The lipid peroxidation processes in patients with ulcerous illness of stomach and duodenum // Clin. Lab. Diagnostics. - 1998. - Vol. 6. - P. 10-14 (In Russian).
9. Halliwell B. Free radicals, antioxidants and human disease: Curiosity, cause or consequence. - Lancet, 1994. - Vol. 344. - P. 721-724.
10. Harikrishnan R., Balasundaram Ch., Moon Y.-G., Kim M.-Ch., Kim J.-S., Dharaneedharan S., Heo M-S. Phytotherapy of ulcerative dermatitis induced by Aeromonas hydrophila infection in goldfish (Carassius auratus) // Acta Vet. Hung. - 2010. - Vol. 58. - P. 29-37.
11. Hayes J.D., McLellan L.I. Glutathione and glutathione-dependent enzymes represent a coordinately regulated defence against oxidative stress // Free Rad. - 1999. - Res., 31, P. 273-300.
12. Kamyshnikov V.S. Reference book on clinic and biochemical researches and laboratory diagnostics, MEDpress-uniform, Moscow. - 2004. - (In Russian).
13. Kane A.S., Dykstra M.J., Noga E.J., Reimschuessel R., Baya A., Driscoll C., Paerl H.W., Landsberg J. Etiologies, observations and reporting of estuarine finfish lesions // Marine Environ. Res. - 2000. - P. 473-477.
14. Khoo L. Fungal diseases in fish // Sem. Avian Exotic Pet. Med. - 2000 - Vol. 9. P. 102-111.
15. Koroliuk M.A., Ivanova L.I., Majorova I.G., Tokarev V.E. A method of determining catalase activity // Lab. Delo. - 1988. - Vol. 1. - P. 16-19 (In Russian).
16. Kostiuk V.A., Potapovich A.I., Kovaleva Zh.V. A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation // Vopr. Med. Khim. - 1990 - Vol. 36 (2). - P. 88-91 (In Russian).
17. Kurhalyuk N., Tkachenko H. Induction of oxidative stress and antioxidant defenses in the liver of sea trout, Salmo trutta L., with ulcerative dermal necrosis // Arch. Pol. Fish. -2011. - Vol. 19. - P. 229-240.
18. Kurhalyuk N., Palczynska K., Tkachenko H. Oxidative stress and antioxidant defenses in reproductive organs of brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis // 25th Congress of the Polish physiological society. September 15-17,
2011, Olsztyn, Poland / In: J. Physiol. Pharmacol. - 2011. - 62(Suppl. 1). - P. 147.
19. Kurhalyuk N., Palczynska K., Tkachenko H., Wnuk Lipinska P., Szornak M., Wnuk Lipinski M., Goryl P., Jurga J., Zawistowska M., Kopp-Ostrowski K., Romanowski M. Bilans aktywnosci enzymow antyoksydacyjnych u osobnikow troci w^drownej (Salmo trutta m. truttaL.) z wrzodziej^c^ martwic^ skory. (Balance of antioxidant defence system of brown trouts affected by ulcerative dermal necrosis) // In book: “Przeobrazenia i zmiany”. Pod red. R. Droby, W. Nowakowskiego, D. Uchmana, K. Starczewskiego. Nauki przyrodnicze, nauki scisle, wiedza interdyscyplinarna. - Siedlce: Wydawnictwo Akademii Podlaskiej. - 2010. - P. 97-107.
20. Kurhalyuk N., Tkachenko H., Palczynska K. Antioxidant enzymes profile in the brown trout (Salmo trutta trutta) with ulcerative dermal necrosis // Bull. Vet. Inst. Pulawy. -2009. - Vol. 53. - P. 813-818.
21. Kurhalyuk N., Tkachenko H., Palczynska K. Lipid peroxidation and antioxidant defense system in spawn of brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis // Arch. Pol. Fish. - 2010. - Vol. 18. - P. 115-122.
22. Kurhalyuk N., Tkachenko H., Palczynska K. Resistance of erythrocytes from brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome // Pol. J. Vet. Sciences. - 2011. - Vol. 14(3). - P. 443-448.
23. Kurhalyuk N., Tkachenko H., Palczynska K., Wysocka-Lipinska N. Wplyw wrzodziej^cej martwicy skory na bilans antyoksydacyjny ikry i mlecza troci w^drownej (Salmo trutta m. trutta L.) z dorzecza Slupi w okresie tarla. (The influence of ulcerative dermal necrosis on antioxidant defence system of spawn and milt of brown trout (Salmo trutta m. trutta L.) from basin of river Slupia during spawning) // Slupskie Prace Biologiczne. - 2011. - Vol.8. - P. 73-88.
24. Kurhalyuk N., Tkachenko H., Szornak M., Kaminski P. Ekofizjologiczne zaleznosci parametrow bilansu antyoksydacyjnego u troci w^drownej (Salmo trutta m. trutta L.) z wrzodziej^c^ martwic^ skory. In book: “Globalizacja a problematyka ochrony srodowiska”, red. T. Nocha, A. Wesolowskiej. _ Gdansk: Wydawnictwo Gdanskiej Wyzszej Szkoly Administracji. _ 2010. _ P. 418_433.
25. Law M. Differential diagnosis of ulcerative lesions in fish. Environ. Health Perspect. _ 2001._ Vol. 109 (5). _ P. 681_686.
26. Levine R. L., Garland D., Oliver C. N., Amici A., Climent I., Lenz A.-G., Ahn B.-W., Shaltiel S., Stadtman E. R. Determination of carbonyl content in oxidatively modified proteins // Methods Enzymol. _ 1990. _ Vol. 186. _ P. 465_478.
27. Moin V.M. A simple and specific method for determining glutathione peroxidase activity in erythrocytes. Lab. Delo. _ 1986. _ Vol. 12. _ P. 724_727 (In Russian).
28. Munro A.L.S. Ulcerative dermal necrosis, a disease of migratory salmonid fishes in the rivers of the British Isles // Biol. Conserv. _ 1970. _ Vol. 2. _ P. 129_132.
29. Noga E.J. Skin ulcers in fish: Pfiesteria and other etiologies // Toxicol. Pathol. _ 2000. _ Vol. 28(6). _ P. 807_823.
30. Pryor W.A. Oxy-radicals and related species: their formation, lifetimes and reactions. Annu. Rev. Physiol. _ 1986. _ Vol. 48. _ P. 657_667.
31. Ravin H.A. An improved colorimetric enzymatic assay of ceruloplasmin // J. Lab. Clin. Med. _ 1961. _ Vol. 58. _ P. 161_168.
32. Rehulka J. Aeromonas causes severe skin lesions in rainbow trout (Oncorhynchus mykiss): clinical pathology, haematology and biochemistry // Acta Vet. Brno. _ 2002. _ Vol. 71. _ P. 351_360.
33. Roberts R.J. Ulcerative dermal necrosis (UDN) in wild salmonids // Fish Res. _ 1993. — 17. _ P. 3_14.
34. Sagara Y., Dargusch R., Chambers D., Davis J., Schubert D., Maher P. Cellular mechanisms of resistance to chronic oxidative stress // Free Radic. Biol. Med.. _ 1998. _ Vol. 24. _ P. 1375_1389.
35. Sohal R.S. Role of oxidative stress and protein oxidation in the aging process. Free Radic. Biol. Med. _ 2002. _ Vol. 33. _ P. 37_44.
36. Stadtman E.R., Levine R.L. Protein oxidation // Ann. N. Y. Acad. Sci. _ 2000. _ Vol. 899. _P. 191_208.
37. Szewczyk E.: Bacteriological diagnostic // Warszawa, PWN (In Polish). _ 2005.
38. Tkachenko H., Kurhalyuk N., Palczynska K. Responses of antioxidant status in the gills of brown trout (Salmo trutta m. trutta L.) // J. Ecol. Prot. Coastline. _ 2011. _ Vol. 15. _ P. 149_162.
39. Ursini F., Bindoli A. The role of selenium peroxidases in the protection against oxidative damage of membranes. Chem. Phys. Lipids. _ 1987. _ Vol. 44. _ P. 255_276.
40. Valavanidis A., Vlahogianni T., Dassenakis M., Scoullos M. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants // Ecotoxicol. Environ. Saf. _ 2006. _ Vol. 64. _ P. 178_189.
41. Wdzieczak J., Zalesna G., Wujec E., Peres G. Comparative studies on superoxide dismutase, catalase and peroxidase levels in erythrocytes and livers of different freshwater and marine fish species // Comp. Biochem. Physiol. B. _ 1982. _ Vol. 73. _ P. 361_365.
42. Zar J.H. Biostatistical Analysis. 4th ed., Prentice-Hall Inc., Englewood Cliffs, New Jersey. _ 1999.